gap43  (Novus Biologicals)

Journal: Annals of Neurosciences

Article Title: Cyclodextrin-enabled δ-Tocotrienol for Treating Stress and Memory Dysfunction: Molecular Insights, Biotechnological Applications and Biomanufacturing Implications

doi: 10.1177/09727531261479841

Figure Lengend Snippet: Primer Sequences Used for Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and Quantitative Polymerase Chain Reaction (qPCR).

Article Snippet: Protein samples (20 μM) were resolved by SDS-PAGE, transferred to nitrocellulose membrane (Millipore) by electroblotting using a semidry transfer blotter and probed with antibodies including MAP2 (M3696; Sigma-Aldrich), PSD (MA1-046; Thermo Fisher Scientific), NeuF-H/NF-200 (N4142; Sigma-Aldrich), GFAP (G9269; Sigma-Aldrich), GAP43 (sc-17790; Santa Cruz), NCAM (sc-106; Santa Cruz), Vimentin (sc-6260; Santa Cruz), Survivin (71G4B7; Cell Signalling), Nestin (sc-23927; Santa Cruz) and Mortalin.

Techniques: Reverse Transcription, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Sequencing

Journal: Annals of Neurosciences

Article Title: Cyclodextrin-enabled δ-Tocotrienol for Treating Stress and Memory Dysfunction: Molecular Insights, Biotechnological Applications and Biomanufacturing Implications

doi: 10.1177/09727531261479841

Figure Lengend Snippet: Effect of Delta-Tocotrienol (δ-T3) on Markers of Differentiation at the Transcriptional Level in C6 and PC12 Cells. (A) Representative Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Gel Images of mRNA Expression of Neuronal Markers in Control (Dimethylsulfoxide [DMSO]) and δ-T3-treated C6 and PC12 Cells. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) was Used as an Internal Standard. (B, C) Quantitation of RT-PCR Analysis in C6 Cells (B) and PC12 Cells (C) Exposed to DMSO or δ-T3 (5 µM). Gene Expression is Indicated as Relative mRNA Levels to GAPDH, Normalised and Expressed as a Percentage Relative to Control Cells (Assigned 100%). Bars Represent Mean ± SEM of Three Independent Experiments. * p < .05, ** p < .01, *** p < .001; ns = Not Significant.

Article Snippet: Protein samples (20 μM) were resolved by SDS-PAGE, transferred to nitrocellulose membrane (Millipore) by electroblotting using a semidry transfer blotter and probed with antibodies including MAP2 (M3696; Sigma-Aldrich), PSD (MA1-046; Thermo Fisher Scientific), NeuF-H/NF-200 (N4142; Sigma-Aldrich), GFAP (G9269; Sigma-Aldrich), GAP43 (sc-17790; Santa Cruz), NCAM (sc-106; Santa Cruz), Vimentin (sc-6260; Santa Cruz), Survivin (71G4B7; Cell Signalling), Nestin (sc-23927; Santa Cruz) and Mortalin.

Techniques: Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Expressing, Control, Quantitation Assay, Analysis, Gene Expression

Journal: Frontiers in Neuroscience

Article Title: A pH-responsive and self-healing CEC/PEG-(CHO) 2 hydrogel for controlled neuropeptide S delivery and endogenous neural repair after spinal cord injury

doi: 10.3389/fnins.2026.1933351

Figure Lengend Snippet: Phenotypic characterization of mouse NSPCs and the Pro-neurogenic effects of NPS loaded CEC/PEG-(CHO) 2 hydrogel scaffold In Vitro. (a) After 7 days in proliferation medium, isolated NSPCs formed neurospheres. Scale bar = 100 μm. (b–d) Neurospheres were positive for the NSPC markers Nestin, SOX2, and PAX6. Scale bar = 100 μm. (e–g) Quantification of Nestin + , SOX2 + , and PAX6 + cells. (h) Schematic diagram of NSPC isolation. (i) Schematic of the NSPC differentiation assay. (j) After 7 days of differentiation, immature neurons were immunostained for Tuj-1. Scale bar = 50 μm. (k) After 14 days, mature neurons were immunostained for MAP2. Scale bar = 50 μm. (l,m) Quantification of Tuj-1 + immature neurons (l) and MAP2 + mature neurons (m) . ** P < 0.01, *** P < 0.001. (n,o) qPCR analysis of transcription factors associated with neuronal differentiation at 7 (n) and 14 (o) days. * P < 0.05, ** P < 0.01, *** P < 0.001. (p) Schematic of the NSPC axon growth assay. (q) After 7 days in proliferation culture, axons were immunostained for GAP43. Scale bar = 200 μm. (r) Quantification of axon length. (s) qPCR analysis of transcription factors regulating axon growth. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following primary antibodies were used: Nestin (rabbit, Proteintech, 1:400; NSPCs), SOX2 (rabbit, Proteintech, 1:400; NSPCs), PAX6 (rabbit, Proteintech, 1:400; NSPCs), TUJ1 (mouse, Proteintech, 1:400; immature neurons), MAP2 (mouse, Abcam, 1:400; mature neurons), GFAP (rat, Thermo Fisher, 1:400; astrocytes), GAP43 (rabbit, Proteintech, 1:400; axons), MMP-9 (rabbit, Abclonal, 1:400; secreted MMP-9), TNF-α (mouse, Proteintech, 1:400; pro-inflammatory cytokines), IL-10 (mouse, Proteintech, 1:400; anti-inflammatory cytokines), CD68 (rat, Abcam, 1:400; activated immune cells), CSPGs (mouse, Cell Signaling Technology, 1:400; glial scar), synaptophysin (SYP, rabbit, Proteintech, 1:400; presynaptic membranes), and cAMP (rabbit, Proteintech, 1:400; intracellular second messenger).

Techniques: In Vitro, Isolation, Differentiation Assay, Analysis, Growth Assay

Journal: Materials Today Bio

Article Title: Injectable anti-inflammatory, antioxidant supramolecular nanofiber hydrogel for peripheral nerve injury repair and neuropathic pain relief

doi: 10.1016/j.mtbio.2026.102780

Figure Lengend Snippet: Histological analysis of regenerated sciatic nerves 28 days post-PNI in rats. (A) Representative cross-sectional images: HE staining (scale bar: 20 μm), TB staining (scale bar: 20 μm), Gap43 immunohistochemistry (scale bar: 20 μm), S100β immunohistochemistry (scale bar: 20 μm). (B) TEM image representation and local magnification of myelin regeneration (scale: 2 μm). (C) Quantification of average Gap43-positive area (%) in the mid-region of regenerated nerves (n = 4). (D) Quantification of average S100β-positive area in the mid-region of regenerated tissues (n = 4). (E) Statistical analysis of myelin thickness (n = 4). (F) Representative Western blot images of total Gap43, S100β and GAPDH protein expression injured nerves. (G, H) Quantitatively analyze the expression level of S100β and Gap43 were analyzed using ImageJ software (n = 4). Data are presented as the means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

Article Snippet: The antibodies used and their dilution ratio include: NF-κB (CST, 1:1000), P- NF-κB (CST, 1:1000), Gap43 (CST, 1:1000), S100β (Abcam, 1:1000), GAPDH (Proteintech, 1:1000).

Techniques: Staining, Immunohistochemistry, Western Blot, Expressing, Software